1 1

THUNDER™ Phospho-SMAD3 (S423/S425) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-SMAD3 (S423/S425) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-SMAD3P-2500 Brand: Bioauxilium
Share:
Price:
$5,441 USD
$5,441 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Host Mouse
Components


Eu-phospho-SMAD3
Acc-phospho-SMAD3
SMAD3 control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SMAD3 (S423/S425) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with TGFβ1 stimulates phosphorylation of SMAD3 at S423/S425.
Inhibition of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with SB 431542 inhibits phosphorylation of SMAD3 at S423/S425 by TGFβ1.
Z'-factor determination in A549 cells
The Z’-factor value of 0.89 indicates that the assay is robust and suitable for HTS.
A549 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.