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THUNDER™ Extreme Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit

THUNDER™ Extreme Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-XTM-ERKP Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium
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Regular price $409 USD
Regular price Sale price $409 USD
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Product Details

Product Specification


Host Mouse
Antigen ERK1/2
Reactivity Human, Mouse
Components


Eu-phospho-XTMERK1/2
Acc-phospho-XTMERK1/2
ERK1/2 control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail

Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of low levels of Phospho-ERK1/2 (T202/Y204) in cell lysates. This high-sensitivity kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER

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STIMULATION OF PHOSPHO-ERK1/2 (T202/Y204) IN HEK293 CELLS
Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204.
INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN H358 CELLS
Data show that treatment of H358 cells with RMC-4550 inhibits basal phosphorylation of ERK1/2 at T202/Y204.
INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN MCF7 CELLS
Data show that treatment of MCF7 cells with L779,450 inhibits basal phosphorylation of ERK1/2 at T202/Y204.
INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN B LYMPHOCYTES
Data show that treatment of B lymphocyte cells with L779,450 inhibits basal phosphorylation of ERK1/2 at T202/Y204.