Stat4 Recombinant Rabbit mAb (S-4916-70)

Catalog Number: S0B60482 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl 1ml

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Product Details

Product Specification

Host	Rabbit
Antigen	Stat4
Synonyms	Signal transducer and activator of transcription 4; STAT4
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	Q14765
Clone Number	S-4916-70
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	Karpas-299, F9, mouse testis, rat testis
Purification	Protein A
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:400	Hu, Ms, Rt

Background

Stat4 (signal transducer and activator of transcription 4) is an important member of the STAT protein family, encoded by the STAT4 gene located on human chromosome 2, and plays a central regulatory role in the immune system. This protein possesses a typical STAT family structure, containing key functional domains including a DNA-binding domain and an SH2 domain. The primary function of STAT4 is to mediate interleukin-12 (IL-12) signaling; upon IL-12 stimulation, it is phosphorylated and activated by JAK kinases, subsequently forming homodimers and translocating into the nucleus to initiate the transcription of target genes such as interferon-gamma (IFN-γ), thereby driving the differentiation of naive CD4+ T cells into Th1 cells, which is critical for defense against intracellular pathogen infections. In addition, STAT4 also plays an indispensable role in the functional regulation of various immune cells including NK cells and dendritic cells. Its functional abnormalities are closely associated with multiple autoimmune diseases, and STAT4 gene polymorphisms (such as the rs7574865 locus) have been confirmed to significantly increase susceptibility to rheumatoid arthritis and systemic lupus erythematosus; meanwhile, its role in tumor development is also receiving increasing attention, with its high expression in colorectal cancer being positively correlated with tumor invasion depth.

Picture

Western Blot

WB result of Stat4 Recombinant Rabbit mAb
Primary antibody: Stat4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Karpas-299 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 86 kDa

WB result of Stat4 Recombinant Rabbit mAb
Primary antibody: Stat4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: F9 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 86 kDa
This blot was developed with high sensitivity substrate

WB result of Stat4 Recombinant Rabbit mAb
Primary antibody: Stat4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Negative control: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 86 kDa

WB result of Stat4 Recombinant Rabbit mAb
Primary antibody: Stat4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat testis lysate 20 µg
Negative control: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 86 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human testis. Anti-Stat4 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Stat4 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cardiac muscle. Anti-Stat4 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Stat4 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti-Stat4 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti-Stat4 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat testis. Anti-Stat4 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat cardiac muscle. Anti-Stat4 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.