WB result of SLFN5 Rabbit pAb
Primary antibody: SLFN5 Rabbit pAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: T-47D whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 101 kDa
Observed MW: 100 kDa
SLFN5 Rabbit Polyclonal Antibody
SLFN5 Rabbit Polyclonal Antibody
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$70.00 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SLFN5 |
| Synonyms | Schlafen family member 5 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | Q08AF3 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Positive Sample | A549, T-47D, LNCaP, A431 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
SLFN5 (Schlafen family member 5) is a protein-coding gene that belongs to the Schlafen family, which is involved in various biological processes such as cell cycle regulation, immune response, and viral infection. SLFN5 has been identified as a regulator of cell motility and invasion in renal cell carcinoma, and its expression is associated with poor prognosis in colorectal adenocarcinoma. Additionally, SLFN5 acts as a restriction factor against herpes simplex virus type 1 (HSV-1) infection by suppressing viral transcription. It is also regulated by the androgen receptor in castration-resistant prostate cancer.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in A431 cells. Anti- SLFN5 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
