WB result of RBPMS Recombinant Rabbit mAb
Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-937 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Low expression control: U-937 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22-28 kDa
This blot was developed with high sensitivity substrate
RBPMS Recombinant Rabbit mAb (S-2387-163)
RBPMS Recombinant Rabbit mAb (S-2387-163)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RBPMS |
| Synonyms | RNA-binding protein with multiple splicing; RBP-MS; Heart and RRM expressed sequence (Hermes); HERMES |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q93062 |
| Clone Number | S-2387-163 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, A549, A431, HeLa, mouse heart, rat heart |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
| IF | 1:500 | Ms |
Background
RBPMS (RNA-binding protein with multiple splicing) is a vertebrate-conserved RNA-binding protein that harbors a single, ~80-aa RNA-recognition motif (RRM) flanked by N- and C-terminal extensions rich in prolines, is expressed as numerous mRNA and protein isoforms from a >230-kb locus, and functions as a master post-transcriptional regulator by directing alternative splicing, stability and translation of target mRNAs critical for cardiac, smooth-muscle, retinal ganglion cell and oocyte development, as well as for DNA-damage responses through interactions with RIF1 and modulation of TGF-β/BMP signaling pathways.
Picture
Picture
Western Blot
WB result of RBPMS Recombinant Rabbit mAb
Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22-28 kDa
This blot was developed with high sensitivity substrate
WB result of RBPMS Recombinant Rabbit mAb
Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22-28 kDa
This blot was developed with high sensitivity substrate
Immunocytochemistry
ICC shows positive staining in A549 cells. Anti- RBPMS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse retina. Anti- RBPMS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
