Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cells) labeled with Rat IgG1 Isotype Control antibody at 1/20 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Rat IgG1 Isotype control (S-3599)
Rat IgG1 Isotype control (S-3599)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Clone Number | S-3599 |
| Antibody Type | Rat mAb |
| Isotype | IgG1 |
| Application | FCM, ICFCM |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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