WB result of Rabbit Anti-Mouse IgG (H+L) mAb
Primary antibody: Rabbit Anti-Mouse IgG (H+L) mAb at 1/5000 dilution
Lane 1: mouse serum lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25, 55 kDa
Observed MW: 26, 50 kDa
Rabbit Anti-Mouse IgG (H+L)
Rabbit Anti-Mouse IgG (H+L)
Price:
Regular price
$70 USD
Regular price
Sale price
$70 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Mouse IgG |
| Immunogen | Native protein |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ELISA, WB, FCM |
| Reactivity | Ms |
| Purification | Protein A |
| Concentration | 5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ELISA | 1:5000-1:10000 | Ms |
| WB | 1:5000-1:10000 | Ms |
| FCM | 1:500 | Ms |
Picture
Picture
Western Blot
FC
Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, left) / U266B1 (Human multiple myeloma B lymphocyte, right) labeled with SDT Mouse Anti-Human CD70 antibody at 1/200 dilution (1 μg) (S0B5570) followed by Rabbit Anti-Mouse IgG (H+L) mAb at 1/500 dilution (1 μg). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the tertiary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
