WB result of PODXL Recombinant Rabbit mAb
Primary antibody: PODXL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A-204 whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: Caco-2 whole cell lysate 20 µg
Negative control: A204 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 160-250 kDa
PODXL Recombinant Rabbit mAb (S-2014-9)
PODXL Recombinant Rabbit mAb (S-2014-9)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PODXL |
| Synonyms | Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1 (PC; PCLP-1); PCLP; PCLP1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | O00592 |
| Clone Number | S-2014-9 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HT-29, HeLa, Caco-2, mouse kidney |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:5000 | Hu, Ms, Rt |
Background
Podocalyxin-like protein 1 (PODXL) is a type I transmembrane protein belonging to the CD34 family of sialomucins, typically expressed in kidney podocytes, vascular endothelial cells, hematopoietic progenitors, mesothelium, and certain neurons. It has a highly glycosylated extracellular domain, a single transmembrane domain, and a short cytoplasmic tail. PODXL functions primarily as an anti-adhesive molecule, regulating cell morphology and adhesion, and is crucial for kidney development and function. In cancer, PODXL is often overexpressed and associated with aggressive tumor phenotypes, poor prognosis, and potentially chemoresistance.
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Picture
Western Blot
WB result of PODXL Recombinant Rabbit mAb
Primary antibody: PODXL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 150-170 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
