WB result of Phospho-Src Family (Tyr416) Recombinant Rabbit mAb
Primary antibody: Phospho-Src Family (Tyr416) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated A431 whole cell lysate 20 µg
Lane 2: A431 treated with 50 mM Pervanadate for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
Phospho-Src Family (Tyr416) Recombinant Rabbit mAb (S-1556-29)
Phospho-Src Family (Tyr416) Recombinant Rabbit mAb (S-1556-29)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Src Family (Tyr416) |
| Synonyms | Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; SRC1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P12931 |
| Clone Number | S-1556-29 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | A431 treated with Pervanadate, NIH/3T3 treated with Pervanadate |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
| ICC | 1:500 | Hu |
Background
Phospho-Src Family (Tyr416) refers to the Src family of protein tyrosine kinases when phosphorylated at the tyrosine residue 416. This post-translational modification is crucial for the activation of Src kinases, which include Src, Lyn, Fyn, Yes, Lck, Blk, and Hck. Phosphorylation at Tyr416, located within the activation loop of the kinase domain, upregulates enzyme activity, while phosphorylation at another regulatory site, Tyr527, downregulates it. In the context of cancer, Src activity is often dysregulated, contributing to tumorigenesis and cancer progression. Understanding the mechanisms of Src activation, such as phosphorylation at Tyr416, is important for developing targeted therapies against cancer.
Picture
Picture
Western Blot
WB result of Phospho-Src Family (Tyr416) Recombinant Rabbit mAb
Primary antibody: Phospho-Src Family (Tyr416) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 10 mM Pervanadate for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
Dot Blot
Dot blot result of Phospho-Src Family (Tyr416) Recombinant Rabbit mAb
Lane1: Src famly (Tyr416) phospho peptide
Lane2: Src famly (Tyr416) unmodified peptide
Primary antibody: Phospho-Src Family (Tyr416) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunocytochemistry
ICC analysis of A431 cells treated with pervanadate (50mM, 5min) (top panel) and A431 cells untreated with pervanadate (50mM, 5min) (below panel). Anti- Phospho-Src Family (Tyr416) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
