WB result of Akt (pan) Rabbit mAb
Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
Lane 1: GST-Akt1 protein 10 ng
Lane 2: GST-Akt2 protein 10 ng
Lane 3: GST-Akt3 protein 10 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 60~100 kDa
Phospho-Akt (Ser473) Antibody Duo
Phospho-Akt (Ser473) Antibody Duo
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Accession | P31751、 Q9Y243、 P31749 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 |
Background
AKT protein, also known as Akt protein kinase B (PKB), is a serine/threonine-specific protein kinase that plays an important role in various cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration. AKT is particularly significant in cancer research as its overactivation can promote the survival and proliferation of various cancer cells. AKT participates in the PI3K/Akt signaling pathway and regulates various cellular functions such as metabolism, growth, proliferation, survival, transcription, and protein synthesis when fully activated. AKT can be activated by various upstream signals, including integrin, receptor tyrosine kinases (RTKs), B and T cell receptors, and G protein-coupled receptors. Conversely, protein phosphatase 2A (PP2A) can dephosphorylate and deactivate AKT.
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Contents & Protocol
Western Blot
WB result of Akt (pan) Rabbit mAb
Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Lane 5: NIH/3T3 whole cell lysate 20 µg
Lane 6: C2C12 whole cell lysate 20 µg
Lane 7: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
IP
Phospho-Akt (Ser473) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Akt (Ser473) in 0.4 mg NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg (Input)
Lane 2: Phospho-Akt (Ser473) Rabbit mAb IP in NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate
Predicted MW: 56 kDa
Observed MW: 60 kDa
