Perilipin-1 Recombinant Rabbit mAb (S-3093-31)

Catalog Number: S0B60410 Application: WB, IHC-P Reactivity: Ms Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	Perilipin-1
Synonyms	Lipid droplet-associated protein; Perilipin A; Peri; Plin; Plin1
Immunogen	Synthetic Peptide
Location	Endoplasmic reticulum
Accession	Q8CGN5
Clone Number	S-3093-31
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Ms
Positive Sample	differentiated 3T3-L, mouse testis
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Ms
IHC-P	1:2000	Ms

Background

Perilipin-1 is a lipid droplet-associated protein encoded by the PLIN1 gene, predominantly expressed in adipocytes of white and brown adipose tissue. This protein consists of 522 amino acids with a molecular weight of approximately 56 kDa, and is localized on the surface of intracellular neutral lipid droplets. It serves as a "molecular coat" on lipid droplets, inhibiting the lipolytic action of hormone-sensitive lipase (HSL) by directly coating the droplets, thereby protecting stored neutral lipids from excessive breakdown. As a key regulator of lipid droplet dynamics and energy homeostasis, phosphorylation of Perilipin-1 is a central mechanism regulating its function—for example, dephosphorylation at Ser511 can modulate lipid droplet size, while BAP31 protein regulates lipid droplet metabolism by influencing its proteasomal degradation. Dysfunction of this protein is closely associated with various human diseases: C-terminal frameshift mutations in the PLIN1 gene can cause familial partial lipodystrophy, characterized by peripheral fat loss, severe hypertriglyceridemia, and insulin resistance in patients. However, studies have also shown that loss-of-function variants in PLIN1 are associated with lower metabolic risk in the population, exhibiting protective features such as elevated HDL cholesterol, reduced triglycerides, and potentially decreased risk of myocardial infarction and hypertension.

Picture

Western Blot

WB result of Perilipin-1 Recombinant Rabbit mAb
Primary antibody: Perilipin-1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: differentiated 3T3-L whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 62 kDa

WB result of Perilipin-1 Recombinant Rabbit mAb
Primary antibody: Perilipin-1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 62 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse brown fat. Anti-Perilipin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse white fat. Anti-Perilipin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti-Perilipin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse brain. Anti-Perilipin-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.