PCK1 Rabbit Polyclonal Antibody

Catalog Number: S0B60477 Application: WB, IHC-P, IF Reactivity: Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl 1ml

Quantity:

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Product Details

Product Specification

Host	Rabbit
Antigen	PCK1
Synonyms	Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK-C; Serine-protein kinase PCK1; PEPCK1
Immunogen	Synthetic Peptide
Location	Cytoplasm, Endoplasmic reticulum
Accession	P35558
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P, IF
Reactivity	Ms, Rt
Purification	Immunogen Affinity
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Ms, Rt
IHC-P	1:500	Ms, Rt
IF	1:500	Ms, Rt

Background

PCK1, also known as phosphoenolpyruvate carboxykinase 1, is a crucial enzyme involved in gluconeogenesis and the conversion of oxaloacetate to phosphoenolpyruvate. It plays a significant role in maintaining glucose homeostasis by facilitating the production of glucose from non-carbohydrate sources. This enzyme is primarily expressed in tissues such as the liver and kidneys, where gluconeogenesis predominantly occurs. Abnormal regulation or activity of PCK1 has been implicated in metabolic disorders, including diabetes.

Picture

Western Blot

WB result of PCK1 Rabbit pAb
Primary antibody: PCK1 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse adipose lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Lane 4: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 69 kDa

WB result of PCK1 Rabbit pAb
Primary antibody: PCK1 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 69 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-NTSR1 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-NTSR1 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-NTSR1 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunofluorescence

IF shows positive staining in paraffin-embedded mouse kidney. Anti-PCK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded rat liver. Anti-PCK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.