WB result of Thrombin Rabbit mAb
Primary antibody: Thrombin Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70kDa
Observed MW: 75kDa
Thrombin Recombinant Rabbit mAb (SDT-293-58)
Thrombin Recombinant Rabbit mAb (SDT-293-58)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SerpinC1/Antithrombin III |
| Synonyms | N/A |
| Immunogen | Recombinant Protein |
| Location | Secreted, Extracellular space |
| Accession | P00734 |
| Clone Number | SDT-293-58 |
| Antibody Type | Recombinant mAb |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Tag | N/A |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 |
Background
Thrombin is a Na+-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Binding of Na+ is the major driving force behind the procoagulant, prothrombotic and signaling functions of the enzyme, but is dispensable for cleavage of the anticoagulant protein C. The anticoagulant function of thrombin is under the allosteric control of the cofactor thrombomodulin [PMID: 18329094].
Picture
Picture
Western Blot
WB result of Thrombin Rabbit mAb
Primary antibody: Thrombin Rabbit mAb at 1/1000 dilution
Lane 1: Human serum lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70kDa
Observed MW: 80kDa
