Flow cytometric analysis of human peripheral blood cells labeling Pacific Blue Mouse IgG2a, k Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Pacific Blue Mouse IgG2a, κ Isotype Control (S-844-17)
Pacific Blue Mouse IgG2a, κ Isotype Control (S-844-17)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Clone Number | S-844-17 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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FC
4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) was stained with SDT Pacific Blue Mouse IgG2a, k Isotype Control Antibody at 1 μg/test (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
