IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-P2ry12 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
P2ry12 Recombinant Rabbit mAb,PBS Only (SDT-3134-52)
P2ry12 Recombinant Rabbit mAb,PBS Only (SDT-3134-52)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | P2ry12 |
| Synonyms | P2Y purinoceptor 12; P2Y12; ADP-glucose receptor (ADPG-R); P2T(AC); P2Y(AC); P2Y(cyc); P2Y12 platelet ADP receptor (P2Y(ADP)); SP1999; HORK3; P2RY12 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q9H244 |
| Clone Number | SDT-3134-52 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
Background
P2RY12 (purinergic receptor P2Y, G-protein coupled, 12) is a 22 kDa Gαi-coupled seven-transmembrane receptor that is best known as the primary platelet receptor for ADP, where it triggers Rap1- and αIIbβ3 integrin-dependent aggregation and is the target of widely used antithrombotic drugs such as clopidogrel; beyond hemostasis, the same receptor is expressed on osteoclasts, microglia, vascular smooth-muscle cells and certain neurons, mediating ADP-evoked bone resorption, microglial chemotaxis and synaptic pruning, foam-cell formation in atherosclerosis, and neuroinflammatory responses that can either exacerbate or protect against neurodegeneration depending on context.
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Picture
Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-P2ry12 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human glioblastoma. Anti-P2ry12 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
