WB result of OGDH Mouse mAb
Primary antibody: OGDH Mouse mAb at 1/2500 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 116 kDa
OGDH Mouse mAb
OGDH Mouse mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | OGDH |
| Synonyms | 2-oxoglutarate dehydrogenase complex component E1; E1o; HsOGDH; OGDC-E1; OGDH-E1; 2-oxoglutarate dehydrogenase; mitochondrial; Alpha-ketoglutarate dehydrogenase (Alpha-KGDH-E1); Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase |
| Location | Nucleus, Mitochondrion |
| Accession | Q02218 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse heart, rat heart |
| Purification | Protein A |
| Concentration | 1.3 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:100-1:500 | Hu, Ms, Rt |
| ICC | 1:200-1:500 | Hu, Ms, Rt |
Background
OGDH is the core subunit of the α-ketoglutarate dehydrogenase complex (OGDC) in the mitochondrial matrix. As one of the key rate-limiting enzymes of the tricarboxylic acid (TCA) cycle, it catalyzes the oxidative decarboxylation of α-ketoglutarate to succinyl-CoA and NADH, connecting two important metabolic nodes in the cycle and playing a critical role in cellular energy metabolism and redox balance. This enzyme is encoded by the OGDH gene, which is located on human chromosome 7 and encodes a precursor protein with an N-terminal mitochondrial targeting sequence that is processed to form the functional mature enzyme. Structurally, the OGDH protein consists of three domains: the N-terminal domain is responsible for binding the coenzyme thiamine pyrophosphate (TPP) to support substrate decarboxylation, the central region mediates homodimer formation, and the C-terminus contains the interaction interface for binding to dihydrolipoamide dehydrogenase (E3). Due to its central role in metabolism, OGDH activity is regulated by multiple mechanisms, including substrate availability, product inhibition, and phosphorylation modification. At the pathological level, OGDH dysfunction is closely associated with various neurological disorders; studies have found that its activity is significantly reduced in brain tissues of patients with Alzheimer's disease and Parkinson's disease, representing an important mechanism by which impaired energy metabolism contributes to neurodegeneration. In addition, OGDH gene mutations have also been linked to neurodevelopmental disorders such as early-onset dystonia and Leigh syndrome. In recent years, OGDH has also been recognized as a metabolic vulnerability in multiple tumors, and its inhibition represents a therapeutic strategy for targeting tumor metabolism.
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Picture
Western Blot
WB result of OGDH Mouse mAb
Primary antibody: OGDH Mouse mAb at 1/2500 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 116 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-OGDH antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-OGDH antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-OGDH antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-OGDH antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-OGDH antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-OGDH antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
