NTSR1 Recombinant Rabbit mAb (S-4844-38)

Catalog Number: S0B60476 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl 1ml

Quantity:

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Product Details

Product Specification

Host	Rabbit
Antigen	NTSR1
Synonyms	Neurotensin receptor type 1; NT-R-1; NTR1; High-affinity levocabastine-insensitive neurotensin receptor; NTRH; NTRR
Immunogen	Synthetic Peptide
Location	Cell membrane
Accession	P30989
Clone Number	S-4844-38
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	Jurkat, MCF7, PC-3, DU 145, mouse colon, rat colon, COS7
Purification	Protein A
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:4000	Hu

Background

NTSR1 (neurotensin receptor type 1) is a high-affinity G protein-coupled receptor (GPCR) encoded by the NTSR1 gene, belonging to the seven-transmembrane protein family, with its gene localized to the q13.33 region of human chromosome 20. This protein is the primary effector receptor for the neuropeptide neurotensin (NTS), mediating various physiological functions through NTS binding, including blood pressure regulation, thermoregulation, antinociception (analgesia), feeding behavior, as well as intestinal motility and secretion. At the level of signal transduction, upon binding with NTS, NTSR1 activates multiple downstream signaling pathways, including PLC-mediated intracellular calcium release, the PKC/RAF-1/MAPK-ERK1/2 cascade, and the PI3K/AKT pathway, and the activation of these signaling networks in turn regulates cell proliferation, survival, migration, and cytoskeletal dynamics. Precisely because of its strong pro-proliferative and pro-migratory capabilities, NTSR1 plays a tumor-promoting role in various malignancies, with its expression being upregulated in multiple tumors including glioma, pancreatic ductal adenocarcinoma, gastric cancer, and prostate cancer, and is closely associated with poor patient prognosis and enhanced tumor aggressiveness. In addition, NTSR1 is also genetically linked to central nervous system disorders such as schizophrenia, drug addiction, and Parkinson's disease, and its functional abnormalities also involve susceptibility to alcohol and opioid dependence. In recent years, three-dimensional structures of NTSR1 in complex with G proteins have been resolved using techniques such as cryo-electron microscopy, providing an important foundation for in-depth understanding of its allosteric regulatory mechanisms and biased signal transduction (such as biasing toward the β-arrestin pathway to attenuate psychostimulant effects), making it a hotspot target in drug development and tumor-targeted therapy.

Picture

Western Blot

WB result of NTSR1 Recombinant Rabbit mAb
Primary antibody: NTSR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 μg
Lane 2: MCF7 whole Cell lysate 20 μg
Lane 3: PC-3 whole Cell lysate 20 μg
Lane 4: DU 145 whole Cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
This blot was developed with high sensitivity substrate

WB result of NTSR1 Recombinant Rabbit mAb
Primary antibody: NTSR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse colon lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
This blot was developed with high sensitivity substrate

WB result of NTSR1 Recombinant Rabbit mAb
Primary antibody: NTSR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat colon lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
This blot was developed with high sensitivity substrate

WB result of NTSR1 Recombinant Rabbit mAb
Primary antibody: NTSR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-NTSR1 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-NTSR1 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-NTSR1 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.