Flow cytometric analysis of human PBMCs (human peripheral blood mononuclear cells) labeled with Mouse IgG2b, κ Isotype Control antibody at 1/50 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Mouse mAb IgG2b Isotype Control
Mouse mAb IgG2b Isotype Control
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Clone Number | S-5677 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | WB, IHC-P, ICC, FCM, IP, IF, ICFCM |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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Picture
FC
Flow cytometric analysis of human PBMCs (human peripheral blood mononuclear cells) fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Mouse IgG2b, κ Isotype Control antibody at 1/50 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
