| Host | Rabbit |
| Antigen | LDH-C/A |
| Synonyms | L-lactate dehydrogenase C chain, Cancer/testis antigen 32 (CT32), LDH testis subunit, LDH testis subunit, LDH-X, LDH3, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, LDH muscle subunit (LDH-M), Renal carcinoma antigen NY-REN-59, PIG19 |
| Location | Cytoplasm |
| Accession | P07864、 P00338 |
| Clone Number | S-R328 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
| application | dilution | species |
| WB | 1:1000-1:2000 | null |
| IHC-P | 1:8000 | null |
Lactate dehydrogenase (LDH) is mainly responsible for catalyzing the conversion of pyruvate into lactate. It is present in almost all living cells. It consists of four subunits forming a tetramer and can form five different subtypes of LDH. Their enzymatic properties are similar, but there are obvious specific differences in tissue distribution. LDH-M and LDH-H subunits are encoded by the LDHA and LDHB genes and can also be expressed as LDHA and LDHB. LDHC is the testis-specific LDH protein.
Primary antibody: LDH-C/A Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 34 kDa
Primary antibody: LDH-C/A Rabbit mAb at 1/2000 dilution
Lane 1: mouse liver lysate 5 µg
Lane 2: mouse testis lysate 5 µg
Negative control: mouse liver lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 34 kDa
Exposure time: 30 sIHC shows positive staining in paraffin-embedded human testis. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
