WB result of Invivo rat IgG2a isotype control, anti-trinitrophenol
Primary antibody: Invivo rat IgG2a isotype control, anti-trinitrophenol at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Invivo rat IgG2a isotype control, anti-trinitrophenol
Invivo rat IgG2a isotype control, anti-trinitrophenol
Price:
Regular price
$120.00 USD
Regular price
Sale price
$120.00 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rat |
| Clone Number | S-R483 |
| Antibody Type | Rat mAb |
| Isotype | Rat IgG2a, κ |
| Application | WB, IHC-P, FC, In vivo control |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95% Determined by SDS-PAGE |
| Endotoxin | <2EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:500 | |
| FCM | 1:500 |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
Picture
Picture
Western Blot
WB result of Invivo rat IgG2a isotype control, anti-trinitrophenol
Primary antibody: Invivo rat IgG2a isotype control, anti-trinitrophenol at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
WB result of Invivo rat IgG2a isotype control, anti-trinitrophenol
Primary antibody: Invivo rat IgG2a isotype control, anti-trinitrophenol at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
FC
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling rat IgG2a isotype control antibody at 1/500 dilution (1 μg) / (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunohistochemistry
IHC shows negative staining in paraffin-embedded mouse kidney. rat IgG2a isotype control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded mouse spleen. rat IgG2a isotype control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
