WB result of IFITM3 Rabbit pAb
Primary antibody: IFITM3 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 15 kDa
IFITM3 Rabbit Polyclonal Antibody
IFITM3 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IFITM3 |
| Synonyms | Interferon-induced transmembrane protein 3; Dispanin subfamily A member 2b (DSPA2b); Interferon-inducible protein 1-8U |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Lysosome, Endosome, Cell membrane |
| Accession | Q01628 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa, HepG2, THP-1 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
Background
Interferon-induced transmembrane protein 3 (IFITM3) is a small transmembrane protein that plays a crucial role in various cellular functions. It is ubiquitously expressed in most cell types, primarily localized in endosomal and lysosomal membranes, and can also be found in plasma and endoplasmic reticulum membranes. IFITM3 is best known for its antiviral activity, as it can inhibit the entry of a broad spectrum of viruses, including influenza A virus, Zika virus, coronaviruses, and others, by preventing viral membrane fusion with host cells. In addition to its antiviral function, IFITM3 is involved in cell adhesion, stem cell migration, and immune regulation. It has also been implicated in tumor development and progression, as well as in the pathogenesis of diseases like Alzheimer's Disease. The expression of IFITM3 is regulated by interferons, and genetic variations in the IFITM3 gene have been associated with susceptibility to viral infections.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-IFITM3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-IFITM3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-IFITM3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-IFITM3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
