Schematic diagram of Human Plasma cells Panel Kit.
Human Plasma cells Panel Kit
Human Plasma cells Panel Kit
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$420 USD
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Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human peripheral blood spiked with U266B1 cells |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This three‑color panel is designed for detecting B cells and plasma cells in human peripheral blood. CD19 was included as a general B‑cell marker to identify the B‑cell population, although it is important to note that CD19 expression is downregulated on terminally differentiated plasma cells. CD38 is a marker often used to detect activated B cells and plasma cells, as it is highly expressed on plasmablasts and plasma cells. CD138 (Syndecan-1) is a classic marker of plasma cells, but its expression may be weak or lost in some plasma cells (especially some myeloma cells). Therefore, strong CD38 positivity is often used as an important supplement for plasma cell gating. The expression pattern of CD38 and CD138 can identify plasma cells as CD38+CD138+. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
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FC
Human peripheral blood spiked with U266B1 cells were stained using the Human Plasma cells Panel Kit consisting of anti-human CD19 FITC, anti-human CD38 Alexa Fluor® 647 and anti-human CD138 PE conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 777 (S0B88805) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD19 FITC-A vs SSC-A dot plot show the Mature B cells and apply the CD45+ gate.
4. Create a CD138 PE-A vs CD38 Alexa Fluor® 647-A dot plot and apply the CD19- gate. Create gates to encompass CD138+CD38+ cells (Plasma cells), CD38+CD138- cells (Activated B cells /plasmablasts), and CD38-CD138- cells (Resting mature B cells)
5. Create a CD138 PE-A vs CD38 Alexa Fluor® 647-A dot plot and apply the CD19+ gate. Create gates to encompass CD38+CD138- cells (activated B cells/plasmablasts, requiring additional markers such as CD27 for definitive identification) and CD38-CD138- cells (resting mature B cells).
