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Human NNMT Protein, His Tag

Human NNMT Protein, His Tag

Catalog Number: S0A0194 Brand: Starter
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$548.00 SGD
$548.00 SGD
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Product Details

Product Specification


Species Human
Synonyms Nicotinamide N-methyltransferase, NNMT
Accession P40261
Amino Acid Sequence

Protein sequence (P40261, Met1-Leu264, with C-His Tag) MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL
Expression System E.coli
Molecular Weight Predicted MW: 31.3 kDa Observed MW: 30 kDa
Purity ＞95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Unconjugated
Physical Appearance Lyophilized powder
Storage Buffer

Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4 with 3% trehalose.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

Background

Nicotinamide N-methyltransferase (NNMT) is a cytoplasmic enzyme that catalyzes the methylation of nicotinamide (vitamin B3) using S-adenosyl methionine (SAM) as the methyl donor, producing S-adenosylhomocysteine (SAH) and 1-methylnicotinamide (MNA). Its overexpression is implicated in various cancers, metabolic disorders, and aging, as it consumes SAM and influences global epigenetic patterns by altering the SAM:SAH ratio. NNMT is now recognized as a key metabolic enzyme with potential as a therapeutic target for cancer and obesity.

Picture

SDS-PAGE

2μg（R: reducing conditions）