Standard curve
Example of Human IL-18 standard curve in Assay Diluent #1L.
Human IL-18 OneStep ELISA Kit
Human IL-18 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-18 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 2.4%； Inter-assay: 2.9% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 50.897 pg/mL |
| Range | 156.25 pg/mL – 10000 pg/mL |
| Recovery | Serum: 97%, Plasma: 89%, Cell culture supernatant: 101% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
Interleukin-18 (IL-18), a proinflammatory cytokine primarily produced by macrophages, plays a complex role in immune regulation by potently inducing IFN-γ production and, in synergy with IL-12, shifting B-cell responses away from IgE. Its activity is activated by caspase-1 and inhibited by IL-18 binding protein. Paradoxically, IL-18 can also promote IgE production in certain contexts, highlighting its dual function in allergic diseases, while in rheumatoid arthritis, it synergizes with IL-12 and IL-15 to sustain pathogenic Th1 responses and monokine production, underscoring its significance as a therapeutic target.
Picture
Picture
ELISA
Standard curve
Example of Human IL-18 standard curve in Assay Diluent #1BS.
Linearity
The concentrations of Human IL-18 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: human serum (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1916.26 pg/mL in human serum.
The sample is undiluted samples are as follows: Human plasma-EDTA (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1025.40 pg/mL in human plasma-EDTA.
The sample is undiluted samples are as follows: human A431 cell lysate was 50fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 76702.28 pg/mL in human A431 cell lysate.
To assess the linearity of the assay, two samples were spiked with high concentrations of Human IL-18 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, sample was spiked with high concentrations of Human IL-18 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Protocol Diagram
