Standard curve
Example of human GM-CSF standard curve in Assay Diluent #1.
Human GM-CSF OneStep ELISA Kit
Human GM-CSF OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | GM-CSF |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 4.2%； Inter-assay: 5.7% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.8 pg/ml |
| Range | 3.125 pg/ml - 200 pg/ml |
| Recovery | Cell culture supernatant 88% Serum 94% Plasma 95% |
| Assay time | 60 minutes |
| Species reactivity | Human |
| CROSS REACTIVITY | / |
| INTERFERENCE | / |
| NIBSC convert | / |
Background
GM-CSF is a hematopoietic growth factor secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. GM-CSF binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and GM-CSF.
Picture
Picture
ELISA
Linearity
The concentrations of GM-CSF were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 95 pg/mL in stimulated human PBMC supernatant.
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Protocol Diagram
