WB result of H+/K+-ATPase Recombinant Rabbit mAb
Primary antibody: H+/K+-ATPase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse heart lysate 20 μg
Lane 2: unboiled mouse stomach lysate 20 μg
Negative control: unboiled mouse heart lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 114 kDa
Observed MW: 100 kDa
H+/K+-ATPase Recombinant Rabbit mAb (S-3474-110)
H+/K+-ATPase Recombinant Rabbit mAb (S-3474-110)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | H+/K+-ATPase |
| Synonyms | Potassium-transporting ATPase subunit beta; Gastric H(+)/K(+) ATPase subunit beta; Proton pump beta chain; ATP4B |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P51164 |
| Clone Number | S-3474-110 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Rb, Dg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:500 | Ms, Rt |
Background
ATP4B (ATPase H+/K+ transporting subunit beta) is a 291-amino-acid, heavily glycosylated transmembrane protein that forms the regulatory β-subunit of the heterodimeric gastric H+/K+-ATPase proton pump, which exchanges cytoplasmic H+ for extracellular K+ across the apical membrane of parietal cells to drive gastric acid secretion; it stabilizes and targets the catalytic α-subunit to the membrane, acts as a conformational ratchet that locks the pump in its outward-facing E2 state, and contributes to epithelial cell polarity and adhesion through an immunoglobulin-like C-terminal ectodomain .
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti- H+/K+-ATPase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-H+/K+-ATPase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cardiac muscle. Anti- H+/K+-ATPase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-H+/K+-ATPase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cardiac muscle. Anti- H+/K+-ATPase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse stomach. Anti- H+/K+-ATPase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded mouse cardiac muscle. Anti- H+/K+-ATPase antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat stomach. Anti- H+/K+-ATPase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded rat cardiac muscle. Anti- H+/K+-ATPase antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
