WB result of Goat Anti-Rat IgG(H+L) (HRP Conjugate)
Primary antibody: PAX5 Rat mAb (S0B0263) at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 45 kDa
Goat Anti-Rat IgG(H+L) (HRP Conjugate)
Goat Anti-Rat IgG(H+L) (HRP Conjugate)
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$120 USD
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Product Details
Product Details
Product Specification
| Host | Goat |
| Antigen | Rat IgG |
| Antibody Type | Polyclonal antibody |
| Application | WB, IHC-P |
| Reactivity | Rt |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:10000 | Rt |
| IHC-P | 1:2000 | Rt |
Picture
Picture
Western Blot
IP
IHC shows positive staining in paraffin embedded mouse testis. EWSR1/EWS Recombinant Rat mAb (S0B0280) was used at 1/250 dilution, followed by Goat Anti-Rat IgG(H+L) (HRP Conjugate), 1/2000 dilution. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin embedded rat testis. EWSR1/EWS Recombinant Rat mAb (S0B0280) was used at 1/250 dilution, followed by Goat Anti-Rat IgG(H+L) (HRP Conjugate), 1/2000 dilution. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
