WB result of
Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot)
(HRP Conjugate)
Primary antibody: α-tubulin Mouse mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 52 kDa
Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (HRP Conjugate)
Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (HRP Conjugate)
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Product Details
Product Details
Product Specification
| Host | Goat |
| Antigen | Mouse IgG |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | ELISA, WB |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| ELISA | 1:10000 | |
| WB | 1:10000 |
Background
This antibody has been tested by ELISA to ensure minimal cross-reaction with human, Bovine, Horse serum proteins.
Picture
Picture
Western Blot
WB result of
Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot)
(HRP Conjugate)
Primary antibody: α-tubulin Mouse mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 52 kDa
WB result of
Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot)
(HRP Conjugate)
Primary antibody: α-tubulin Mouse mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 52 kDa
