Human peripheral blood mononuclear cells were stained with Alexa Fluor® 647 Mouse Anti-Human CD14 antibody and either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (Left panel) or SDT Mouse Anti-Human CD64 antibody (Right panel) at 1 μg/test (S0B0910). Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (Alexa Fluor® 488 Conjugate) at 1/2000 dilution was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (Alexa Fluor® 488 Conjugate)
Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (Alexa Fluor® 488 Conjugate)
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Product Details
Product Details
Product Specification
| Host | Goat |
| Antigen | Mouse IgG |
| Antibody Type | Polyclonal antibody |
| Application | ICC, FCM, IF |
| Reactivity | Ms |
| Purification | Immunogen Affinity |
| Concentration | Lot specific* (generally 1.5 to 2 mg/ml) |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:500 | Ms |
| IF | 1:500 | Ms |
| FCM | 1:2000 | Ms |
Background
This antibody has been tested to ensure minimal cross-reaction with human, Bovine, Horse serum proteins.
Picture
Picture
FC
Immunofluorescence
IF shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti- CD45RA antibody (S0B0626) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human anaplastic large cell lymphoma. Anti- CD45RA antibody (S0B0626) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human tonsil. Anti- CD45RA antibody (S0B0626) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
