WB result of FRS2-α Recombinant Rabbit mAb
Primary antibody: FRS2-α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 μg
Lane 2: A204 whole cell lysate 20 μg
Lane 3: PC-3 whole cell lysate 20 μg
Lane 4: SH-SY5Y whole cell lysate 20 μg
Lane 5: HeLa whole cell lysate 20 μg
Negative control: Ramos whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 70 kDa
This blot was developed with high sensitivity substrate
FRS2-α Recombinant Rabbit mAb (S-4065-59)
FRS2-α Recombinant Rabbit mAb (S-4065-59)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FRS2-α |
| Synonyms | Fibroblast growth factor receptor substrate 2; FGFR-signaling adaptor SNT; Suc1-associated neurotrophic factor target 1 (SNT-1); FRS2 |
| Immunogen | Synthetic Peptide |
| Location | Membrane |
| Accession | Q8WU20 |
| Clone Number | S-4065-59 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | A204, PC-3, SH-SY5Y, HeLa, 3T3-L1, C2C12, PC-12, C6, COS7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
FRS2-α (fibroblast growth factor receptor substrate 2-α, also known as SNT1) is a critical lipid-anchored adapter protein in the FRS2 family that serves as a core mediator in downstream signal transduction of fibroblast growth factor receptors (FGFRs) and neurotrophic factor receptors (such as TRK). Its molecular structure includes a myristoylation sequence responsible for anchoring to the cell membrane, a phosphotyrosine-binding (PTB) domain that binds the juxtamembrane region of receptors, and a tyrosine-rich C-terminal region. Upon receptor activation, FRS2-α undergoes tyrosine phosphorylation, and specific tyrosine sites in its C-terminus (such as Tyr196 and Tyr436) subsequently serve as docking platforms for recruiting SH2 domain-containing signaling proteins (such as the Grb2-Sos complex and the protein tyrosine phosphatase SHP-2), thereby activating key signaling pathways such as Ras-MAPK to transduce extracellular signals to the nucleus for regulating cell growth, proliferation, and differentiation. In addition, its function is subject to negative feedback regulation mediated by MAPK, and it plays important roles in developmental biology (such as ocular development) and RET oncogene signal transduction, with broad tissue distribution but higher expression levels in organs such as the brain and kidney.
Picture
Picture
Western Blot
WB result of FRS2-α Recombinant Rabbit mAb
Primary antibody: FRS2-α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 3T3-L1 whole cell lysate 20 μg
Lane 2: C2C12 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 68 kDa
WB result of FRS2-α Recombinant Rabbit mAb
Primary antibody: FRS2-α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 μg
Lane 2: C6 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 68 kDa
This blot was developed with high sensitivity substrate
WB result of FRS2-α Recombinant Rabbit mAb
Primary antibody: FRS2-α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 68 kDa
