Flow cytometric analysis of IL-10 expression in C57BL/6 mouse splenocytes CD4+ T cells stimulated with Invivo anti-mouse CD3ε Recombinant mAb (S0B0661, 10 μg/ml for palate coating) and NA/LE Syrian Hamster anti-mouse CD28 mAb (S0B0003E, 2 μg/ml for palate coating) alone with IL-2(10 ng/ml for palate coating) and IL-4(15 ng/ml for palate coating). The cells were then harvested and restimulated for 6 hr with PMA and Ionomycin in the presence of Monensin (right Panel) or unstimulated (left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with SDT FITC Rat Anti-Mouse IL-10 Antibody at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Rat Anti-Mouse IL-10 Antibody (S-R643)
FITC Rat Anti-Mouse IL-10 Antibody (S-R643)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IL-10 |
| Synonyms | Interleukin-10; Cytokine synthesis inhibitory factor (CSIF); Il-10; Il10 |
| Location | Secreted |
| Accession | P18893 |
| Clone Number | S-R643 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | ICFCM |
| Reactivity | Ms |
| Positive Sample | Th2 polarized C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1μg per million cells in 100μl volume | Ms |
Background
Interleukin-10 (IL-10) is an 18 kDa anti-inflammatory cytokine composed of 160 amino acids folded into two intrachain disulfide-linked α-helical dimers that signals through the heterotetrameric IL-10R1/IL-10R2 receptor complex to activate JAK1/TYK2-mediated STAT3 phosphorylation, thereby down-regulating major histocompatibility class II, co-stimulatory molecules and pro-inflammatory cytokines such as TNF-α, IL-1, IL-6, IL-8, IL-12 and IFN-γ, while simultaneously up-regulating IL-1RA and soluble TNF receptors, ultimately exerting potent immunosuppressive and tissue-protective functions in both innate and adaptive immunity.
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