Epigen Protein, Human
Epigen Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Epigen, EPGN, EPG, Epigen precursor |
| Accession | Q6UW88 |
| Amino Acid Sequence | Ala24-Ala95 |
| Expression System | E.coli |
| Molecular Weight | 8kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 150mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1、Freed D M. et al. (2017) EGFR Ligands Differentially Stabilize Receptor Dimers to Specify Signaling Kinetics. Cell. 171(3):683-695. 2、Kochupurakkal B S. et al. (2005) Epigen, the last ligand of ErbB receptors, reveals intricate relationships between affinity and mitogenicity. J Biol Chem. 280(9): 8503-8512. |
Background
Epigen, human (EPGN) is a member of the epidermal growth factor family. EPGN is ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration. EPGN Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases. The precursor of EPG is a widely expressed transmembrane glycoprotein that undergoes cleavage at two sites to release a soluble EGF-like domain. EPGN might represent the last EGF-like growth factor and define a category of low affinity ligands, whose bioactivity differs from the more extensively studied high affinity ligands. Recombinant EPGN is expressed by Escherichia coli, composed of 72 (Ala24-Ala95) amino acids.
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Bioactivity
SDS-PAGE
