EphA10 His Tag Protein, Human

Catalog Number: UA010609 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE

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$722.00 SGD

Size: 100μg 500μg

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Product Details

Product Specification

Species	Human
Antigen	EphA10
Synonyms	Ephrin type-A receptor 10, EPHA10
Accession	Q5JZY3
Amino Acid Sequence	Glu34-Ala565, with C-terminal 10*His EEVILLDSKASQAELGWTALPSNGWEEISGVDEHDRPIRTYQVCNVLEPNQDNWLQTGWISRGRGQRIFVELQFTLRDCSSIPGAAGTCKETFNVYYLETEADLGRGRPRLGGSRPRKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSRRGFHLAFQDVGACVALVSVRVYYKQCRATVRGLATFPATAAESAFSTLVEVAGTCVAHSEGEPGSPPRMHCGADGEWLVPVGRCSCSAGFQERGDFCEACPPGFYKVSPRRPLCSPCPEHSRALENASTFCVCQDSYARSPTDPPSASCTRPPSAPRDLQYSLSRSPLVLRLRWLPPADSGGRSDVTYSLLCLRCGREGPAGACEPCGPRVAFLPRQAGLRERAATLLHLRPGARYTVRVAALNGVSGPAAAAGTTYAQVTVSTGPGAPWEEDEIRRDRVEPQSVSLSWREPIPAGAPGANDTEYEIRYYEKGQSEQTYSMVKTGAPTVTVTNLKPATRYVFQIRAASPGPSWEAQSFNPSIEVQTLGEAASGSRDQSPAGGGSGGGSHHHHHHHHHH
Expression System	HEK293
Molecular Weight	68-90kDa
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1. Jing Peng, Danhua Zhang. Coexpression of EphA10 and Gli3 promotes breast cancer cell proliferation, invasion and migration, J Investig Med. 2021 Aug;69(6):1215-1221. Epub 2021 May 14. 2. Kullander K., Klein R. Mechanisms and functions of Eph and ephrin signalling. Nat. Rev. Mol. Cell Biol. 2002;3:475-486. 3. Dodelet V.C., Pasquale E.B. Eph receptors and ephrin ligands: Embryogenesis to tumorigenesis. Oncogene. 2000;19:5614-5619. 4. Aasheim H.C., Patzke S., Hjorthaug H.S., Finne E.F. Characterization of a novel Eph receptor tyrosine kinase, EphA10, expressed in testis. Biochim. Biophys. Acta. 2005;1723:1-7. 5. Nagano K., Maeda Y., Kanasaki S., Watanabe T., Yamashita T., Inoue M., Higashisaka K., Yoshioka Y., Abe Y., Mukai Y., Kamada H., Tsutsumi Y., Tsunoda S. Ephrin receptor A10 is a promising drug target potentially useful for breast cancers including triple negative breast cancers. J. Control Release. 2014;189:72-79.

Background

Ephrin receptors (Ephs), the largest subfamily of receptor tyrosine kinases, are known to modulate cell–cell signaling by interacting with cells’ membrane-bound ephrin ligands on neighboring cells, thereby regulating tissue organization, vascular development, and progression of many diseases including cancer. Ephrin receptor A10 (EphA10), a member of the Eph subfamily, is not expressed in normal human adult tissue except in the testis. The Eph/ephrin signaling mediates cell-cell interaction and contributes to the tumorigenesis, angiogenesis and metastasis in many cancers. EphA10 expression is common in malignant cells across histology of various tumors and has been associated with poor prognosis in patients with breast, prostate, and gallbladder cancers.

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SDS-PAGE

1μg (R: reducing conditions, N: non-reducing conditions).

ELISA

Immobilized EphA10 His Tag, Human (Cat. No. UA010609) at 1.0μg/mL (100μL/well) can bind Ephrin-A3 Fc Chimera, Human (Cat. No. UA010141) with EC50 of 0.23-2.35μg/ml.

SPR

Protein A Chip captured Ephrin-A3 Fc Chimera, Human (Cat. No. UA010141), can bind EphA10 His Tag, Human (Cat. No. UA010609) with an affinity constant of 57.08nM as determined in SPR assay.

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EphA10 His Tag Protein, Human

EphA10 His Tag Protein, Human