EPHA1 titration in HTRF assay |
EPHA1 Protein
EPHA1 Protein
Price:
Regular price
$995.00 SGD
Regular price
Sale price
$995.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P21709 |
| Amino Acid Sequence |
T586-D976(end) TDVDREDKLWLKPYVDLQAYEDPAQGALDFTRELDPAWLMVDTVIGEGEFGEVYRGTLRLPSQDCKTVAIKTLKDTSPGGQWWNFLREATIMGQFSHPHILHLEGVVTKRKPIMIITEFMENGALDAFLREREDQLVPGQLVAMLQGIASGMNYLSNHNYVHRDLAARNILVNQNLCCKVSDFGLTRLLDDFDGTYETQGGKIPIRWTAPEAIAHRIFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIEDGYRLPPPVDCPAPLYELMKNCWAYDRARRPHFQKLQAHLEQLLANPHSLRTIANFDPRMTLRLPSLSGSDGIPYRTVSEWLESIRMKRYILHFHSAGLDTMECVLELTAEDLTQMGITLPGHQKRILCSIQGFKD |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 71.2 kDa |
| Purity | >90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Tris, 150mM NaCl, 20mM GSH, 1mM DTT, 10% glycerol, 0.05% Brij-35, pH7.5 |
| Stability & Storage | -80℃ |
Picture
Picture
Bioactivity
The EPHA1 activity was detected using HTRF technology. The reaction was performed by incubating the EPHA1 protein , ATP and substrate at 25℃ for 50 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
SDS-PAGE
SEC-HPLC
