WB result of ENT2 Rabbit pAb
Primary antibody: ENT2 Rabbit pAb at 1/10000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50 kDa
Observed MW: 60~70 kDa
ENT2 Rabbit Polyclonal Antibody
ENT2 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ENT2 |
| Synonyms | Equilibrative nucleoside transporter 2; hENT2; 36 kDa nucleolar protein HNP36; Delayed-early response protein 12; Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter (Equilibrative NBMPR-insensitive nucleoside transporter); Hydrophobic nucleolar protein, 36 kDa; Nucleoside transporter, ei-type; Solute carrier family 29 member 2; SLC29A2; DER12; HNP36 |
| Immunogen | Synthetic Peptide |
| Location | Apical cell membrane |
| Accession | Q14542 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP, ICFCM |
| Reactivity | Hu, Rt |
| Positive Sample | HepG2, K562, Jurkat, SH-SY5Y |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:10000 | Hu |
| IP | 1:50 | Hu |
| IHC-P | 1:250 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
| ICFCM | 1:500 | Hu |
Background
ENT2, or Equilibrative Nucleoside Transporter 2, is a bidirectional transporter embedded in the biological membrane and is found in most tissue and cell types. It plays a crucial role in mediating the uptake of purine and pyrimidine nucleosides and nucleobases, as well as transporting a variety of nucleoside-derived drugs, which are predominantly used in anticancer therapy. The high expression of ENT2 has been correlated with advanced stages of different types of cancers, making it a significant interest as a potential therapeutic target. Furthermore, ENT2 is known to play critical roles in signaling pathways and cell cycle progression.
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Western Blot
FC
Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast) labelling ENT2 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
IP
ENT2 Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ENT2 in 0.4 mg K562 whole cell lysate.
Western blot was performed on the immunoprecipitate using ENT2 Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: K562 whole cell lysate 20 µg (Input)
Lane 2: ENT2 Recombinant Rabbit mAb IP in K562 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in K562 whole cell lysate
Predicted MW: 50 kDa
Observed MW: 60~70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in K562 cells. Anti-ENT2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
