ICC shows positive staining in Histone H3-mCherry-E-tag transfected 293T cells. Anti- E tag (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
E tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-911-14)
E tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-911-14)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | E tag |
| Immunogen | Synthetic Peptide |
| Clone Number | S-911-14 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:500 | Species Independent |
Background
The E tag is an epitope tag with a 13-residue sequence of GAPVPYPDPLEPR. The E tag can help with detection of proteins in western blot, IP, or microscopic techniques.
Picture
Picture
Immunocytochemistry
ICC shows negative staining in vector-transfected 293T cells. Anti- E tag (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
