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DCAF16/DDB1 His Tag Protein, Human

DCAF16/DDB1 His Tag Protein, Human

Catalog Number: UA080546 Brand: UA BIOSCIENCE
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$490 USD
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Product Details

Product Specification


Species Human
Accession Q9NXF7、Q16531
Amino Acid Sequence

G2-L216 end (DCAF16), N-His-TEV
M1-H1140 end (DDB1)
Expression System Baculovirus-InsectCells
Molecular Weight 25.9 KDa/126.9 KDa (~26 KDa/126 KDa)
Endotoxin <1EU/μg
Conjugation Unconjugated
Tag His Tag
Physical Appearance Liquid
Storage Buffer

50 mM Tris-HCl (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT
Stability & Storage

-80℃

Guidelines

Please use rapid thawing with running water to thaw the protein samples.
For complete recovery, mix well and spin before use.
Product must not be stored in diluted solutions.
Aliquots below 10μl are not advisable.
Avoid repetitive freeze-thaw cycles!

Picture

Bioactivity

Lane M: Marker
Lane 1: Bodipy-Ub + UBE1 + Ubch5b + His- DCAF16/DDB1 with ATP
Lane 2: Bodipy-Ub + UBE1+ Ubch5b with ATP
Lane 3: Bodipy-Ub + UBE1+ Ubch5b without ATP
The ubiquitin conjugating activity of His-DCAF16/DDB1 was validated
through its ability to catalyse the generation of polyubiquitin chains in the
presence of the E1 activating enzyme UBE1, the E2 conjugating enzyme
Ubch5b and Bodipy-ubiquitin. Incubation of His-DCAF16/DDB1 for 60
minutes at 37˚C in the presence of Bodipy-ubiquitin, UBE1, Ubch5b and ATP
(Lane 1) was compared alongside two control reactions with either HisDCAF16/DDB1 (Lane 2) or His-DCAF16/DDB1&ATP (Lane 3) excluded
from the reaction. Ubiquitin conjugates were identified by the migration of the
Bodipy-ubiquitin band and these were observed only in the presence of ATP
and His-DCAF16/DDB1.

SDS-PAGE

Lane M: Marker
Lane 1: 2 μg, reduced
Lane 2: 2 μg, non-reduced