Flow cytometric analysis of MCF7 cells labelling Cytokeratin 19 antibody at 1/500 dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 at 1/1000 dilution was used as the secondary antibody.
Cytokeratin 19 Recombinant Rabbit mAb,PBS Only (SDT-107-19)
Cytokeratin 19 Recombinant Rabbit mAb,PBS Only (SDT-107-19)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Cytokeratin 19 |
| Synonyms | KRT19, CK-19 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P08727 |
| Clone Number | SDT-107-19 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, ICC, FCM |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt, 4°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | |
| IHC-P | 1:1000 | |
| FCM | 1:500 | |
| ICC | 1:250 |
Background
Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 is a type I keratin. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically found in the periderm, the transiently superficial layer that envelops the developing epidermis. KRT19 is also known as Cyfra 21-1.Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.
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Validation Data
Western Blot
WB result of Cytokeratin 19 Rabbit mAb
Primary antibody: Cytokeratin 19 Rabbit mAb at 1/500 dilution
Lane 1: Hela whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 39 kDa
Exposure time: 20s
WB result of Cytokeratin 19 Rabbit mAb
Primary antibody: Cytokeratin 19 Rabbit mAb at 1/500 dilution
Lane 1: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 39 kDa
Exposure time: 120 s
WB result of Cytokeratin 19 Rabbit mAb
Primary antibody: Cytokeratin 19 Rabbit mAb at 1/500 dilution
Lane 1: rat kidney whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 39 kDa
Exposure time: 120 s
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon.
Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta.
Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach.
Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney.
Anti-Cytokeratin19 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in MCF7 cells. Anti-Cytokeratin 19 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
