Cys-Protein G
Cys-Protein G
Product Details
Product Details
Product Specification
| Amino Acid Sequence | Leu298-Glu495 with an N-terminal Cys |
| Expression System | E.coli |
| Molecular Weight | Approximately 21.9 kDa, and it migrates with an apparent molecular mass of 40 kDa in SDS-PAGE. |
| Purity | ＞95% by SDS-PAGE or HPLC. |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PB, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | For long term storage, the product should be stored ≤ -20℃. |
Background
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcic. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F (ab') fragments of IgG. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology Recombinant Cys-Protein G is a single non-glycosylated polypeptide chain containing 201 amino acids. and there is an N-terminal cysteine for Site-Specific Conjugation.
