C4d Recombinant Rabbit mAb (SDT-330-2)
C4d Recombinant Rabbit mAb (SDT-330-2)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | C4d |
| Synonyms | N/A |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P0C0L4 |
| Clone Number | SDT-330-2 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.125 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:2500 | |
| IHC-P | 1:1000 |
Background
Complement component 4 (C4), in humans, is a protein involved in the intricate complement system, originating from the human leukocyte antigen (HLA) system. It serves a number of critical functions in immunity, tolerance, and autoimmunity with the other numerous components. Furthermore, it is a crucial factor in connecting the recognition pathways of the overall system instigated by antibody-antigen (Ab-Ag) complexes to the other effector proteins of the innate immune response.C4d is a degradation product of complement activator. If C4d is high in the tumor of patients, its survival period will be reduced; Complement fragment C4d can be used as a biomarker for early diagnosis and prognosis prediction of lung cancer patients.
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Western Blot
WB result of C4d Rabbit mAb Primary antibody: C4d Rabbit mAb at 1/2500 dilution Lane 1: T47D whole cell lysate 20 µg Lane 2: HepG2 whole cell lysate 20 µg Negative control: T47D whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 193kDa Observed MW: 210kDa Exposure time: 90s
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-C4d antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-C4d antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-C4d antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-C4d antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
