WB result of BMP4 Recombinant Rabbit mAb
Primary antibody: BMP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 μg
Lane 2: mouse heart lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 55 kDa
BMP4 Recombinant Rabbit mAb (S-3938-66)
BMP4 Recombinant Rabbit mAb (S-3938-66)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BMP4 |
| Synonyms | Bone morphogenetic protein 4; BMP2B; DVR4 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P12644 |
| Clone Number | S-3938-66 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse skeletal muscle, mouse heart, rat skeletal muscle, rat heart |
| Predicted Reactivity | Bv, Rb |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:200 | Hu, Ms, Rt |
| IF | 1:500 | Ms |
Background
BMP4 (Bone Morphogenetic Protein 4) is a key member of the BMP subfamily within the transforming growth factor-β (TGF-β) superfamily. It is a highly evolutionarily conserved secreted signaling molecule that exerts pleiotropic regulatory functions in embryonic development, organogenesis, and tissue homeostasis maintenance. Its precursor protein is proteolytically cleaved to generate the mature active dimer, which exerts its effects by binding to and activating a heterotetrameric receptor complex composed of BMPR-IA, BMPR-IB, and BMPR-II, subsequently phosphorylating the downstream transcription factors Smad1/5/8. These then form complexes with Smad4 and translocate to the nucleus to regulate target gene expression, while signaling can also be transduced through non-Smad pathways (such as MAPK and PI3K pathways). During embryonic development, BMP4 is one of the earliest critical morphogens involved in establishing the body axis and determining dorsoventral polarity. In the early embryos of vertebrates, the concentration gradient of BMP4 dictates the boundary between neuroectoderm and epidermal ectoderm fates, with its activity finely regulated by antagonists such as Noggin, Chordin, and Follistatin to generate precisely patterned signals. Furthermore, BMP4 promotes osteogenic differentiation and chondrogenesis in skeletal development, and also plays irreplaceable roles in the morphogenesis of organs such as teeth, heart, kidneys, and limbs. In adult tissues, BMP4 participates in bone remodeling, adipocyte differentiation, and iron metabolism regulation. Its dysregulated expression is associated with various diseases, including fibrodysplasia ossificans progressiva (FOP), pulmonary arterial hypertension, tumorigenesis (such as colorectal cancer and prostate cancer), and congenital heart defects. Thus, BMP4 is not only a core research molecule in developmental biology but also an important therapeutic target in the fields of regenerative medicine and disease treatment.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-BMP4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-BMP4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-BMP4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-BMP4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-BMP4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
