WB result of BiP Rabbit pAb
Primary antibody: BiP Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 78 kDa
BiP Rabbit Polyclonal Antibody
BiP Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Endoplasmic reticulum chaperone BiP; 78 kDa glucose-regulated protein (GRP-78); Binding-immunoglobulin protein; Heat shock protein 70 family protein 5 (HSP70 family protein 5); Heat shock protein family A member 5; Immunoglobulin heavy chain-binding protein; HSPA5; GRP78 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P11021 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Xe, Bv, Hm, Sq, Or |
| Purification | Immunogen Affinity |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
The BiP protein, also known as Binding immunoglobulin protein or HSPA5, is a crucial molecular chaperone located in the endoplasmic reticulum (ER) that belongs to the Hsp70 family. BiP binds to hydrophobic amino acid residues of unfolded proteins entering the ER, preventing incorrect folding and aggregation. It also interacts with folded proteins to protect them from aggregation and degradation. Under ER stress conditions, BiP interacts with ER stress sensors such as ATF6, IRE1α, and PERK, regulating their activation and initiating the ER stress response pathway to help cells cope with unfolded protein accumulation. Due to its essential role in the cell, abnormal expression or dysfunction of BiP is closely related to the development of various diseases. For instance, BiP is overexpressed in tumor cells, associated with tumor invasion, metastasis, and drug resistance. Additionally, some genetic and neurodegenerative diseases are linked to abnormal expression or dysfunction of BiP.
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Western Blot
WB result of BiP Rabbit pAb
Primary antibody: BiP Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 78 kDa
WB result of BiP Rabbit pAb
Primary antibody: BiP Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 78 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-BiP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-BiP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-BiP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-BiP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
