SD rat splenocytes were stained with SDT Biotin Mouse IgG2a Isotype Control Antibody (right panel) at 1/20 dilution (1 μg) followed by Sav-PE. Cells without incubation with primary antibody and secondary antibody (left panel) was used as unlabeled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software
Biotin Mouse IgG2a Isotype Control (C1.18.4)
Biotin Mouse IgG2a Isotype Control (C1.18.4)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Clone Number | C1.18.4 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
Picture
Picture
FC
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hela (Human cervix adenocarcinoma epithelial cell) labelling SDT Biotin Mouse IgG2a Isotype Control Antibody at 1/20 dilution (1 μg) (right panel) followed by Sav-iFluor 488 compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
