WB result of APRIL Recombinant Rabbit mAb
Primary antibody: APRIL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: COLO 205 whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 29 kDa
APRIL Recombinant Rabbit mAb (S-2667-23)
APRIL Recombinant Rabbit mAb (S-2667-23)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | APRIL |
| Synonyms | Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand (APRIL); TNF- and APOL-related leukocyte expressed ligand 2 (TALL-2); TNF-related death ligand 1 (TRDL-1); CD256; TALL2; ZTNF2; TNFSF13 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | O75888 |
| Clone Number | S-2667-23 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Rt |
| Positive Sample | Raji, HeLa, COLO 205, HT-29, PC-12 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Rt |
Background
A proliferation-inducing ligand (APRIL), officially designated CD256 and encoded by the TNFSF13 gene, is a type-II transmembrane protein of the tumor necrosis factor (TNF) superfamily that is cleaved into a soluble cytokine; it binds the receptors BCMA and TACI on B cells, thereby driving B-cell maturation, plasma-cell survival in the bone marrow, and class-switch recombination, while also promoting tumor-cell proliferation in multiple myeloma and other B-cell malignancies, and is being pursued therapeutically via neutralizing antibodies or decoy receptors to curb autoimmunity and cancer .
Picture
Picture
Western Blot
WB result of APRIL Recombinant Rabbit mAb
Primary antibody: APRIL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 29 kDa
