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APC Mouse Anti-Human CD14 Antibody (S-3449)

APC Mouse Anti-Human CD14 Antibody (S-3449)

Catalog Number: S0B80885 Application: FCM Reactivity: Hu Conjugation: APC Brand: Starter
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$160 USD
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Product Details

Product Specification


Host Mouse
Antigen CD14
Synonyms Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein
Location Secreted, Cell membrane
Accession P08571
Clone Number S-3449
Antibody Type Mouse mAb
Isotype IgG2a,k
Application FCM
Reactivity Hu
Positive Sample Human peripheral blood cells
Purification Protein A
Concentration 0.1 mg/ml
Conjugation APC
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.09% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 2μl per million cells in 100μl volume Hu

Background

CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein primarily expressed on monocytes, macrophages, and some granulocytes. It functions as a pattern recognition receptor (PRR) and plays a crucial role in the innate immune response by recognizing bacterial lipopolysaccharides (LPS), pathogen-associated molecular patterns (PAMPs), and damage-associated molecular patterns (DAMPs). CD14 exists in two forms: membrane-bound CD14 (mCD14) and soluble CD14 (sCD14), which can be secreted by activated cells. It acts as a co-receptor for Toll-like receptor 4 (TLR4) and enhances the immune response to LPS by facilitating its delivery to the TLR4-MD2 complex. Additionally, CD14 is involved in TLR4-independent signaling pathways and contributes to tissue homeostasis by promoting the clearance of apoptotic cells.

Picture

FC

Flow cytometric analysis of Human CD14 expression on human peripheral blood cells. Human peripheral blood cells were stained with APC Mouse IgG2a, k Isotype Control (left panel) or SDT Mouse Anti-Human CD14 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.