Human PBMC were stained with Alexa Fluor® 700 Rat IgG2b Isotype Control (right panel) at 1/20 dilution (1 μg). Cells without incubation with primary antibody and secondary antibody (left panel) was used as unlabeled control. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 700 Rat IgG2b Isotype Control (S-R486)
Alexa Fluor® 700 Rat IgG2b Isotype Control (S-R486)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Clone Number | S-R486 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b |
| Application | FCM, ICFCM |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
Picture
Picture
FC
Flow cytometric analysis of 4% paraformaldehyde fixed and 0.1% Tween permeabilized human PBMC labeled with Alexa Fluor® 700 Rat IgG2b Isotype Control (right panel) at 1/20 dilution (1 μg). Cells without incubation with primary antibody and secondary antibody (left panel) was used as unlabeled control. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
