Flow cytometric analysis of Mouse CD71 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow were stained with PE Rat Anti-Mouse TER-119 Antibody and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 647 Rat Anti-Mouse CD71 (TfR1) Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse CD71 (TfR1) Antibody (RI7217)
Alexa Fluor® 647 Rat Anti-Mouse CD71 (TfR1) Antibody (RI7217)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD71 |
| Synonyms | Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; Tfrc |
| Location | Cell membrane |
| Accession | Q62351 |
| Clone Number | RI7217 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Isotype Control | S0B8044 |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD71, also known as the transferrin receptor 1 (TfR1), is a type II transmembrane glycoprotein that plays a crucial role in cellular iron uptake by binding to transferrin, which facilitates iron transport into the cell. This receptor is highly expressed on rapidly proliferating cells, including erythroid precursors, activated immune cells, and cancer cells, as these cells require significant iron for DNA synthesis and metabolic processes. In clinical contexts, CD71 is used as a marker for identifying immature red blood cells (such as proerythroblasts and reticulocytes) and is also associated with aggressive tumor growth in various cancers, including pancreatic and colon cancers. Additionally, studies have shown that CD71 expression on neutrophils can be linked to poor prognosis in pancreatic ductal adenocarcinoma (PDAC) patients, indicating its potential as a prognostic biomarker.
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