Flow cytometric analysis of Mouse CD34 expression on C57BL/6 mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with Brilliant Violet 421™ Rat Anti-Mouse Lineage Cocktail antibody and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Left panel) or SDT Alexa Fluor® 647 Rat Anti-Mouse CD34 Antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse CD34 Antibody (RAM34)
Alexa Fluor® 647 Rat Anti-Mouse CD34 Antibody (RAM34)
Price:
Regular price
$160 USD
Regular price
Sale price
$160 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD34 |
| Synonyms | Hematopoietic progenitor cell antigen CD34 |
| Location | Membrane |
| Accession | Q64314 |
| Clone Number | RAM34 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Format | 0101010101 |
| Application | FCM |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD34 is a highly glycosylated type I transmembrane glycoprotein that serves as a key marker for various stem and progenitor cells, particularly hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs). It is selectively expressed on the surface of early-stage HSCs, vascular endothelial cells, and some mesenchymal stem cells. Functionally, CD34 plays a crucial role in mediating cell adhesion and homing of HSCs to the bone marrow through interactions with L-selectin. It also helps maintain the undifferentiated state of progenitor cells and supports their proliferation. In clinical applications, CD34 is widely used to isolate and enrich HSCs for bone marrow transplantation and gene therapy. Additionally, its expression in certain cancers, such as leukemia and some solid tumors, makes it a potential biomarker for cancer diagnosis and a target for therapeutic interventions.
Picture
Picture
FC
