Flow cytometric analysis of CD24 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 Antibody and either Alexa Fluor® 488 Rat IgG2b, κ Isotype Control (left panel) or SDT Alexa Fluor® 488 Rat Anti-Mouse CD24 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 488 Rat Anti-Mouse CD24 Antibody (M1/69)
Alexa Fluor® 488 Rat Anti-Mouse CD24 Antibody (M1/69)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD24 |
| Synonyms | Signal transducer CD24; Lymphocyte antigen 52 (Ly-52); M1/69-J11D heat stable antigen (HSA); Nectadrin; R13-Ag; X62 heat stable antigen; Cd24a; Ly-52; Cd24 |
| Location | Cell membrane |
| Accession | P24807 |
| Clone Number | M1/69 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD24, also known as the cluster of differentiation 24, is a highly glycosylated glycosylphosphatidylinositol (GPI)-anchored surface protein that plays crucial roles in various physiological and pathological processes. Initially identified as a B-cell differentiation antigen, it is widely expressed in hematopoietic stem cells, B and T lymphocytes, epithelial cells, and neural cells. Structurally, CD24 has an intracellular domain, a transmembrane domain, and a heavily glycosylated extracellular domain, which is involved in cell adhesion, migration, differentiation, and apoptosis.
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