Flow cytometric analysis of human peripheral blood cells labeling SDT Alexa Fluor® 488 Mouse IgG2a, k Isotype Control Antibody at 1 μg/test (red line histogram), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabeled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (S-844-17)
Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (S-844-17)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Clone Number | S-844-17 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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Picture
FC
4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell) was stained with SDT Alexa Fluor® 488 Mouse IgG2a, k Isotype Control Antibody at 1 μg/test (right Panel), cells without incubation with primary antibody and secondary antibody was used as unlabeled control. (left Panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
